OCHRATOXIN A EVALUATION IN Theobroma cacao L. "WHITE COCOA" DURING THE HARVESTING PROCESS, FERMENTED, DRIED AND STORED

  • Norma Ramos
  • Américo Castro
  • José Juárez
  • Otilia Acha de la Cruz
  • Nadia Rodríguez
  • Jesús Blancas
  • Jackeline Escudero
  • Jackeline Escudero
  • Arnaldo Navarro
Keywords: Ochratoxin A (OTA), Cacao White Piura, immunoaffinity columns, High Performance Liquid Chromatography (HPLC)

Abstract

The presence of ochratoxinA(OTA), a mycotoxin with carcinogenic properties, was analyzed in the white cocoa of Piura. Samples were collected in four stages of grain production: harvest, fermented, dried and stored. The analysis was done both on the grain shell and on the cotyledon. The samples were collected from the collection center of the Association of Small Producers of Cocoa Piura (APPROCAP). OTA was extracted from the samples using 1% sodium bicarbonate, purified by immunoaffinity columns and then quantified by high performance liquid chromatography (HPLC). A detectable concentration of OTA (1.92 ± 0.02 μg / kg) was found in the fermentation stage. The harvest, drying and storage stages in the cocoa grain production process did not show OTA.

Downloads

Download data is not yet available.

Author Biographies

Norma Ramos

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

Américo Castro

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

José Juárez

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

Otilia Acha de la Cruz

Faculty of Chemical and Textile Engineering, National University of Engineering, Av. Túpac Amaru 210, Lima 25, Peru.

Nadia Rodríguez

Faculty of Chemical and Textile Engineering, National University of Engineering, Av. Túpac Amaru 210, Lima 25, Peru.

Jesús Blancas

Faculty of Chemical and Textile Engineering, National University of Engineering, Av. Túpac Amaru 210, Lima 25, Peru.

Jackeline Escudero

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

Jackeline Escudero

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

Arnaldo Navarro

Faculty of Pharmacy and Biochemistry, National University of San Marcos, Jr. Puno 1002. Botanical Garden. Lima Peru.

Published
2016-12-31