EVALUATION OF ANTIOXIDANT ACTIVITY OF THE STANDARDIZED HYDROALCOHOLIC EXTRACT FROM Juglans neotropica Diels (PERUVIAN WALNUT) LEAVES
Abstract
Juglans neotropica Diels "peruvian walnut" is a tree that grows in jungle, in areas of
montane and montane rain forest whose leaves have antioxidant, astringent, antibacterial and
antiseptic properties. The objective was to determine the antioxidant activity of a standardized
hydroalcoholic extract of Juglans neotropica Diels leaves. Leaves from Huandoval district,
Pallasca province, Ancash Region were used. Standardization analysis included determination
of moisture, physicochemical parameters, solubility march, phytochemical screening, and
thin layer chromatography; antioxidant activity was determined by the DPPH inhibition
assay. In phytochemical screening there was a greater concentration of phenolic compounds
and antioxidant activity was at 25 and 35 μg/mL respectively, with an IC 50 of 12.82 μg/mL.
In conclusion, the standardized hydroalcoholic extract from Juglans neotropica Diels leaves
fulfilled the quality parameters, and showed high antioxidant activity.
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