PHYSICOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT CAPACITY OF VACCINIUM FLORIBUNDUM KUNTH “PUSHGAY” EXTRACT

  • Gaby Espinoza
  • Mónica Guadalupe Retuerto-Figueroa
  • elia Vargas De La Cruz
  • Jossimar Paúl Huamaní Tarazona
  • Marco Rolando Aronés Jara
  • María Rosario Calixto Cotos
  • Eva Ramos Llica
  • Tania Torres Aguilar
  • Arilmi Gorriti Gutiérrez
  • Fabio Espichán
Keywords: antioxidant capacity, screening, DPPH, ABTS, polyphenols, Vaccinium floribundum Kunth

Abstract

The fruit of the Vaccinium floribundum Kunth “pushgay” is part of the group of native berries, which has a large amount of antioxidants, much greater than the blueberries that are currently exported to different countries. The objective of this study was the determination of the physicochemical parameters and the in vitro antioxidant capacity of the plant extract of Vaccinium floribundum Kunth. Extracts were prepared by maceration from freeze-dried fruits, using 50%, 70% and 96% ethanol as a solvent. The extracts presented similar values of relative density, total solids, refractive index, degrees Brix and pH. Phytochemical screening showed the presence of carbohydrates, reducing sugars, tannins, amino compounds and heterosides, highlighting phenolic compounds and flavonoids with relevant antioxidant capacity. The 50% extract had a higher content of total phenols (38,131 ± 0,242 mg GAE/g extract); as well as, greater antioxidant capacity by the DPPH method (TEAC 28089,156 ± 147,482 μg/mL). The 96% extract presented (TEAC 13609,683 ± 21,669 μg/mL). It is concluded that the 50% hydroalcoholic extract of pushgay has greater antioxidant capacity that maintains a correlation with the amount of phenolic compounds with the DPPH method.

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Author Biographies

Marco Rolando Aronés Jara

Departamento de Medicina Humana, Universidad Nacional de San Cristóbal de Huamanga. 05001, Huamanga, Ayacucho, Perú

María Rosario Calixto Cotos

Escuela Profesional de Ingeniería Agroindustrial, Facultad de Química e Ingeniería Química. UNMSM

Eva Ramos Llica

Facultad de Farmacia y Bioquímica-Laboratorio de Farmacognosia, Grupo de Investigación Farmacognosia y Medicina Tradicional, Universidad Nacional Mayor de San Marcos, Lima-Perú

Tania Torres Aguilar

Facultad de Farmacia y Bioquímica-Laboratorio de Farmacognosia, Grupo de Investigación Farmacognosia y Medicina Tradicional, Universidad Nacional Mayor de San Marcos, Lima-Perú.

Arilmi Gorriti Gutiérrez

Facultad de Farmacia y Bioquímica-Laboratorio de Farmacognosia, Grupo de Investigación Farmacognosia y Medicina Tradicional, Universidad Nacional Mayor de San Marcos, Lima-Perú

Fabio Espichán

Universidad Peruana Cayetano Heredia (UPCH), Lima, Perú

Published
2023-06-30