OPTIMIZATION OF THE PROCESS OF IMMOBILIZATION OF LIPASE PRODUCED BY Marinobacter sp CH27 IN OCTADECILO – SEPABEAD

Abstract

The aim of these research was the optimize in the support hydrophobic octadecilo-sepabead by interfacial adsorption, for which the experimental design was used Box-Behnken 3 factors and 3 levels with a total of 12 experiments and 3 replicas on the central point. The variables and their levels selected were: force Ionic (2.5, 5.0 and 10.0 mM), time (30, 60 and 120 min) and pH (6.0; 7.0 and 8.0) and them variable answers were the activity of the enzyme immobilized (U/g support) and the percentage immobilization. The optimum parameters of the immobilization process were: pH 6.62, 30 min and ionic strength 10 Mm. After adjust the parameters to the model polynomial quadratic, is obtained 11,16 U/g support for the activity of lipase immobilized, with 81.50% and 74.0% of activity relative and performance of immobilization respectively.