IMPLEMENTATION OF ANALYTICAL METHODOLOGY FOR QUANTIFICATION OF PROTEINS IN Arthrospira platensis MICROALGAE

  • Leenin Flores Ramos
  • Anthony Ruiz Soto
DOI: https://doi.org/10.37761/rsqp.v83i4.219
Keywords: Spectrophotometry, microalgae, Box Behnken, Youden-Steiner

Abstract

The Hartree-Lowry method was developed and implemented for quantification of protein in Arthorspira platensis microalgae by spectrophotometry UV-Vis. In order to achieve this goal, the protein extraction factors were optimized by Box Behnken design of response surface and the following results of the implementation parameters were obtained: the linearity showed a coefficient of determination (R2 ) greater than 0.995, a coefficient of variation less than 2% for repeatability, a percentage recovery within 94% to 101%, the limit of detection and limit of quantification were 2.36 µg/mL and 7.86 µg/mL respectively, and the robustness showed that some parameters such as sample weight and volume of reagent C presented high influence in the evaluated factors.

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Author Biographies

Leenin Flores Ramos

Instrumental Analysis Laboratory - Aquatic Biotechnology Laboratory, Instituto del Mar del Perú, Esquina Gamarra and General Valle s / n Chucuito Callao, Callao, Peru.

Anthony Ruiz Soto

Instrumental Analysis Laboratory - Aquatic Biotechnology Laboratory, Instituto del Mar del Perú, Esquina Gamarra and General Valle s / n Chucuito Callao, Callao, Peru.

Published
2017-12-31
Issue
Vol. 83 No. 4 (2017): Journal of the Sociedad Química Del Perú
Section
Artículos

Copyright (c) 2017 Sociedad Química del Perú

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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