OPTIMIZATION OF A FRUCTOOLIGOSACCHARIDES PRODUCTIVE PROCESS FROM CAROB EXTRACT USING FRUCTOSILTRANSFERASES

Abstract

Fructooligosaccharides (FOS) are fructose polymers, linked by β-glucosidic bonds (2-1), considered low caloric food sweeteners with prebiotic function and can be obtained from sucrose by biosynthesis. The fruits of the carob tree (Prosopis pallida) contain mainly carbohydrates where sucrose stands out. For this reason, the production of FOS from carob extract was optimized using a pectinase from A. aculeatus. The response surface methodology using was applied to optimize the parameters that affected production: time, temperature and carob extract; then they were verified experimentally. The biosynthesis process of FOS was carried out with carob extract at the concentrations of 20, 35 and 50 ° Bx, Pectinase at 10, 35 and 61mg, at 45, 55 and 65 °C for 0,5, 9,5 and 18 h, with stirring at 150 rpm The analysis methods were glucose oxidase and AOAC 999.03, for glucose and FOS respectively and the Minitab 16 program. The optimal reaction conditions for the production of FOS were: carob extract 38 °Bx, enzyme 31 mg, 54 °C, 150 rpm and 12 h. The experimental yield in the transformed extract was FOS 16, 1% quantified by HPLC.